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目的：利用网络药理学探讨疏风解毒胶囊治疗新型冠状病毒肺炎的机制。方法：通过TCMSP网络平台检索疏风解毒胶囊的活性成分以及作用靶点；通过GeneCards数据库获取COVID-19的相关靶标。由Cytoscape软件和STRING数据库建立药物-化合物-靶标网络、靶标蛋白互作PPI网络。R语言的cluster Profiler工具包对于核心靶点展开GO功能富集以及KEGG通路富集分析。结果：根据筛选条件得到207个化合物和248个药物相应靶点。PPI核心网络包含48个蛋白，关键蛋白涉及MAPK1、MAPK3、STAT1、IL6等。GO功能富集分析取得1 618个条目(P<0.05),其中生物过程(BP)1 498个，细胞组成(CC)33个，分子功能(MF)87个；KEGG通路富集有157条通路与COVID-19相关(P<0.05),涉及人类巨细胞病毒感染、IL-17信号通路、HIF-1信号通路、TNF信号通路等。结论：疏风解毒胶囊中的活性化合物能通过多靶点、多通路治疗新型冠状病毒肺炎。
Abstract：Objective: To explore the mechanism of Shufeng jiedu capsules in the treatment of Corona Virus Disease 2019(COVID-19)based on network pharmacology.Methods: The active compounds and targets of Shufeng jiedu capsules were searched through TCMSP network platform; The targets related to COVID-19 were screened by GeneCards database.The drugs-compounds-targets network and target protein interaction PPI network were constructed through Cytoscape software and STRING database.GO enrichment and KEGG pathway annotation analysis of core targets were performed using cluster Profiler toolkit for R language.Results: According to the screening conditions, 207 compounds and 248 drug targets were obtained.PPI core network contained 48 proteins, the key proteins included MAPK1,MAPK3,STAT1,IL6,etc.GO functional enrichment analysis had 1 618 GO items(P<0.05),including 1 498 biological processes(BP),33 cellular components(CC),87 molecular functions(MF).KEGG pathway enrichment analysis obtained 157 pathways related to COVID-19(P<0.05),involving human cytomegalovirus infection, IL-17 signaling pathway, HIF-1 signaling pathway, TNF signaling pathway, etc.Conclusion: The active compounds in Shufeng jiedu capsules can treat the COVID-19 by multiple targets and multiple pathways.
[1] 毛昀，苏毅馨，薛鹏，等.金花清感颗粒治疗新型冠状病毒肺炎作用机制探讨[J].中药材，2020,43(11):2842-2848.
[2] 陈蕾，谢俊刚.《新型冠状病毒肺炎诊疗方案(试行第七版)》解读[J].医药导报，2020,39(5):613-615.
[3] 张俊雅，徐升.疏风解毒胶囊治疗慢性阻塞性肺疾病急性加重期疗效观察[J].中国中医急症，2019,28(3):505-507.
[4] 李思聪，冯祥，毕磊，等.新型冠状病毒肺炎诊疗方案中成药选用分析与药理研究进展[J].中药材，2020,43(3):764-771.
[5] Guo YR,Cao QD,Hong ZS,et al.The origin,transmission and clinical therapies on coronavirus disease 2019(COVID-19)outbreak-an update on the status[J].Mil Med Res,2020,7(1):11.
[6] 欧亚青，陈璐，边原，等.血浆置换技术在病毒感染类疾病治疗中的应用[J].中南药学，2020,18(4):598-601.
[7] Fonseca Brito L,Brune W,Stahl FR.Cytomegalovirus(CMV)pneumonitis:cell tropism,inflammation,and immunity[J].Int J Mol Sci,2019,20(16):3865.
[8] 周丽萍，王荣丽.HIF-1α及TGF-β1与哮喘关系研究进展[J].临床肺科杂志，2017,22(11):2089-2092.
[9] Lin CC,Lin WN,Cho RL,et al.TNF-α-induced cPLA2 expression via NADPH oxidase/reactive oxygen species-dependent NF-κB cascade on human pulmonary alveolar epithelial cells[J].Front Pharmacol,2016,7:447.
[10] Menegatti S,Bianchi E,Rogge L.Anti-TNF therapy in spondyloarthritis and related diseases impact on the immune system and prediction of treatment responses[J].Front Immunol,2019,10:382.
[11] Lee SY,Yoon BY,Kim JI,et al.Interleukin-17 increases the expression of Toll-like receptor 3 via the STAT3 pathway in rheumatoid arthritis fibroblast-like synoviocytes[J].Immunology,2014,141(3):353-361.
[12] Hsu D,Taylor P,Fletcher D,et al.Interleukin-17 pathophysiology and therapeutic intervention in cystic fibrosis lung infection and inflammation[J].Infect Immun,2016,84(9):2410-2421.
[13] 武明云，虞坚尔，薛征，等.基于MAPK信号通路的中药治疗支气管哮喘的实验研究进展[J].上海中医药大学学报，2019,33(2):86-91.
[14] Duan J,Xie J,Deng T,et al.Exposure to both formaldehyde and high relative humidity exacerbates allergic asthma by activating the TRPV4-p38 MAPK pathway in Balb/c mice[J].Environ Pollu,2020,256:113375.
[15] 何建军.NF-κB、PI3K和STAT1在支气管哮喘患儿外周血单个核细胞中的表达变化及临床意义[J].中国妇幼保健，2018,33(21):4933-4935.
[16] Kumar A,Das S,Agrawal A,et al.Genetic association of key Th1/Th2 pathway candidate genes,IRF2,IL6,IFNGR2,STAT4 and IL4RA,with atopic asthma in the Indian population[J].J Hum Genet,2015,60(8):443-448.
基本信息:
DOI：10.13863/j.issn1001-4454.2020.12.044
中图分类号:R285
引用信息:
[1]李康梅,陈泯锜,李金惠等.疏风解毒胶囊治疗新型冠状病毒肺炎的网络药理学研究[J].中药材,2020,43(12):3080-3087.DOI:10.13863/j.issn1001-4454.2020.12.044.
基金信息:
国家自然科学基金项目(81960794);; 2018年引进博士科研启动基金项目(2018BS007);; 2019年度广西中青年教师科研基础能力提升项目(2019KY0320);; 广西中医药大学2019年研究生教育创新计划重点项目(YCSZ20190017);广西中医药大学2019年研究生教育创新计划一般项目(YCSY20190055)